Abstract A regularization technique based on adversarial perturbation, which was initially developed in the field of image processing, has been successfully applied to text classification tasks and has yielded attractive improvements. We aim to further leverage this promising methodology into more sophisticated and critical neural models in the natural language processing field, i.e., neural machine translation (NMT) models. However, it is not trivial to apply this methodology to such models. Thus, this paper investigates the effectiveness of several possible configurations of applying the adversarial perturbation and reveals that the adversarial regularization technique can significantly and consistently improve the performance of widely used NMT models, such as LSTMbased and Transformer-based models