Abstract. The quick detection of specific substances in objects such as produce items via non-destructive visual cues is vital to ensuring the quality and safety of consumer products. At the same time, it is wellknown that the fluorescence excitation-emission characteristics of many organic objects can serve as a kind of “fingerprint” for detecting the presence of specific substances in classification tasks such as determining if something is safe to consume. However, conventional capture of the fluorescence excitation-emission matrix can take on the order of minutes and can only be done for point measurements. In this paper, we propose a coded illumination approach whereby light spectra are learned such that key visual fluorescent features can be easily seen for material classification. We show that under a single coded illuminant, we can capture one RGB image and perform pixel-level classifications of materials at high accuracy. This is demonstrated through effective classification of different types of honey and alcohol using real images