Abstract We introduce the query-by-example (QBE) paradigm for query answering in the presence of ontologies. Intuitively, QBE permits non-expert users to explore the data by providing examples of the information they (do not) want, which the system then generalizes into a query. Formally, we study the following question: given a knowledge base and sets of positive and negative examples, is there a query that returns all positive but none of the negative examples? We focus on description logic knowledge bases with ontologies formulated in Horn-ALCI and (unions of) conjunctive queries. Our main contributions are characterizations, algorithms and tight complexity bounds for QBE.